The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases

@article{AltaeTran2021TheWI,
  title={The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases},
  author={Han Altae-Tran and Soumya Kannan and F. Esra Demircioglu and Rachel Oshiro and Suchita P. Nety and Luke J. McKay and Mensur Dlaki{\'c} and William P. Inskeep and Kira S. Makarova and Rhiannon K. Macrae and Eugene V. Koonin and Feng Zhang},
  journal={Science},
  year={2021},
  volume={374},
  pages={57 - 65}
}
Description Tracing the origin of CRISPR-Cas CRISPR-Cas systems have transformed genome editing and other biotechnologies; however, the broader origins and diversity of RNA-guided nucleases have largely remained unexplored. Altae-Tran et al. show that three distinct transposon-encoded proteins, IscB, IsrB, and TnpB, are naturally occurring, reprogrammable RNA-guided DNA nucleases (see the Perspective by Rousset and Sorek). In addition to identifying diverse guide-encoding mechanisms, the… 

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