The voltage-sensitive dye RH421 detects a Na+,K+-ATPase conformational change at the membrane surface.

Abstract

RH421 is a voltage-sensitive fluorescent styrylpyridinium dye which has often been used to probe the kinetics of Na+,K+-ATPase partial reactions. The origin of the dye's response has up to now been unclear. Here we show that RH421 responds to phosphorylation of the Na+,K+-ATPase by inorganic phosphate with a fluorescence increase. Analysis of the kinetics… (More)
DOI: 10.1016/j.bbamem.2017.01.022

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Cite this paper

@article{Garcia2017TheVD, title={The voltage-sensitive dye RH421 detects a Na+,K+-ATPase conformational change at the membrane surface.}, author={Alvaro Garcia and Promod R Pratap and Christian L{\"u}pfert and Flemming Cornelius and Denis Jacquemin and Bogdan Lev and Toby W. Allen and Ronald James Clarke}, journal={Biochimica et biophysica acta}, year={2017}, volume={1859 5}, pages={813-823} }