Corpus ID: 977055

The viable but nonculturable state in bacteria.

@article{Oliver2005TheVB,
  title={The viable but nonculturable state in bacteria.},
  author={J. Oliver},
  journal={Journal of microbiology},
  year={2005},
  volume={43 Spec No},
  pages={
          93-100
        }
}
  • J. Oliver
  • Published 2005
  • Biology, Medicine
  • Journal of microbiology
It had long been assumed that a bacterial cell was dead when it was no longer able to grow on routine culture media. We now know that this assumption is simplistic, and that there are many situations where a cell loses culturability but remains viable and potentially able to regrow. This mini-review defines what the "viable but nonculturable" (VBNC) state is, and illustrates the methods that can be used to show that a bacterial cell is in this physiological state. The diverse environmental… Expand

Figures, Tables, and Topics from this paper

Paper Mentions

Interventional Clinical Trial
No one really knows what causes overactive bladder syndrome (OAB). Urinary tract infection (UTI)causes similar symptoms to OAB with the difference being the presence of bacteria, as… Expand
ConditionsOveractive Bladder
InterventionDrug
Recent findings on the viable but nonculturable state in pathogenic bacteria.
  • J. Oliver
  • Biology, Medicine
  • FEMS microbiology reviews
  • 2010
TLDR
The central role of catalase in the VBNC response of some bacteria, including its genetic regulation, is described and a variety of interesting chemical and biological factors have been shown to allow resuscitation, including extracellular resuscitation-promoting proteins, a novel quorum-sensing system and interactions with amoeba. Expand
INTRODUCE OF VIABLE BUT NONCULTURABLE BACTERIA
Viable-But-Nonculturable-State (VBNC) is the condition in which bacteria fail to grow on their routine bacteriological media where they would normally grow and develop into colonies, but are stillExpand
The importance of the viable but non-culturable state in human bacterial pathogens
TLDR
The characteristics of VBNC cells, including the similarities and differences to viable, culturable cells and dead cells, and different detection methods are discussed, and their potential influence on human health is reviewed. Expand
Bacterial resuscitation factors: revival of viable but non-culturable bacteria
TLDR
The importance of understanding this state comes from its similarity to the dormant state of Mycobacterium tuberculosis in the lungs, where the pathogen can remain without symptoms for decades. Expand
Relationship between the Viable but Nonculturable State and Antibiotic Persister Cells
TLDR
It is believed these survival modes represent a continuum between actively growing and dead cells, with VBNC cells being in a deeper state of dormancy than persister cells. Expand
Stepwise changes in viable but nonculturable Vibrio cholerae cells
TLDR
The requirements for resuscitation of VBNC‐state Vibrio cholerae cells were found to change over time, and all cells entered a state from which they could not be resuscitated, even by co‐cultivation with HT‐29. Expand
Current Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria
TLDR
Various aspects of VBNC bacteria are described, which include their proteomic and genetic profiles under the VB NC state, conditions of resuscitation, methods of detection, antibiotic resistance, and observations on Rpf. Expand
Exploring the potential environmental functions of viable but non-culturable bacteria
TLDR
This mini-review will provide new insight for considering the potentially environmental functions of VBNC bacteria, which are a huge microbial resource and could provide important answers to dealing with existing problems of environmental pollution. Expand
The viable but non-culturable state in pathogenic Escherichia coli: A general review
TLDR
Pathogenic and indicator E. coli entering into the VBNC state could have an adverse effect on public health if conventional detection methods are used, where the number of viable cells could be underestimated and theVBNC cells still produce toxins or could, at any time, be resuscitated and become virulent again. Expand
Induction, detection, formation, and resuscitation of viable but non-culturable state microorganisms.
The viable but nonculturable (VBNC) state has been recognized as a strategy for bacteria to cope with stressful environments; in this state, bacteria fail to grow on routine culture medium but areExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 82 REFERENCES
The Public Health Significance of Viable but Nonculturable Bacteria
A bacterium in the viable but nonculturable (VBNC) state is defined here as a cell which fails to grow on the routine bacteriological media on which it would normally grow and develop into a colony,Expand
Formation of Viable but Nonculturable Cells
TLDR
It is realized that some bacteria, in response to certain environmental stresses, may lose the ability to grow on media on which they are routinely cultured, while remaining viable, and is of special concern when considering release into the environment of genetically engineered microorganisms and human pathogens. Expand
The viable but nonculturable state and cellular resuscitation
TLDR
Research, including dilution studies, time required for resuscitation to occur, and the effects of nutrient on recovery, indicates that, at least for V. vulnificus, true resuscitation does occur. Expand
Bacterial viability and culturability.
TLDR
Developments in knowledge of physiological processes in bacteria that may influence the outcome of a culturability test are considered and whether it is appropriate to consider the viability of individual bacteria or whether, in some circumstances, it may be moreappropriate to consider viability as a property of a community of bacteria. Expand
Cell Wall Chemical Composition ofEnterococcus faecalis in the Viable but Nonculturable State
TLDR
Analysis of the biochemical composition of the cell wall of E. faecalis in the VBNC state showed an increase in total cross-linking, and changes were observed in penicillin binding proteins (PBPs), the enzymes involved in the terminal stages of peptidoglycan assembly, and in autolytic enzymes. Expand
Viable but non-culturable and dormant bacteria: time to resolve an oxymoron and a misnomer?
  • M. Barer
  • Biology, Medicine
  • Journal of medical microbiology
  • 1997
TLDR
The proposal that several pathogens which are readily culturable, including Yibrio cholerae, Campylobacter jejuni and Helicobacter pylori, may enter a state in which they retain viability but fail to grow in conventional culture strikes at the core of the equation, viability = culturability. Expand
Viability and activity in readily culturable bacteria: a review and discussion of the practical issues
TLDR
It is argued that failure to differentiate clearly between use of the terms ‘viability’ and ‘culturability” in an operational versus a conceptual sense is fuelling the current debate, and an alternative operational terminology is suggested that replaces ‘VBNC’ with expressions that are internally consistent. Expand
Formation of nonculturable Vibrio vulnificus cells and its relationship to the starvation state
TLDR
The nonculturable response appears to be related to the starvation response, as prestarvation at room temperature for 24 h was found to eliminate the non Culturable response of cells subsequently incubated at 5 degrees C, suggesting that the synthesis of starvation proteins may repress the viable butNonculturable program displayed during low-temperature incubation. Expand
Resuscitation of Vibrio vulnificus from the viable but nonculturable state
TLDR
It is suggested that nutrient is in some way inhibitory to the resuscitation of cells in the VBNC state and that studies which add nutrient in an attempt to detect resuscitation are able to detect only residual culturable cells which might be present and which were not inhibited by the added nutrient. Expand
Induction of Escherichia coli and Salmonella typhimurium into the viable but nonculturable state following chlorination of wastewater.
TLDR
While the effects of chlorine disinfection on Escherichia coli and Salmonella typhimurium in secondary-treated wastewater were examined, it was found that the presence of such nonculturable cells in treated wastewater offers a potential public health hazard. Expand
...
1
2
3
4
5
...