The vault complex

@article{vanZon2003TheVC,
  title={The vault complex},
  author={Arend van Zon and Marieke H. Mossink and Rik J. Scheper and Pieter Sonneveld and Erik A. C. Wiemer},
  journal={Cellular and Molecular Life Sciences CMLS},
  year={2003},
  volume={60},
  pages={1828-1837}
}
Vaults are large ribonucleoprotein particles found in eukaryotic cells. They are composed of multiple copies of a Mr 100,000 major vault protein and two minor vault proteins of Mr 193,000 and 240,000, as well as small untranslated RNAs of 86–141 bases. The vault components are arranged into a highly characteristic hollow barrel-like structure of 35 × 65 nm in size. Vaults are predominantly localized in the cytoplasm where they may associate with cytoskeletal elements. A small fraction of vaults… Expand
Characterization of MVP and VPARP assembly into vault ribonucleoprotein complexes.
TLDR
It took more than 4h for newly synthesized MVPs to be incorporated into vault particles but that biosynthesized VPARPs were completely incorporated into vaults within 1.5h, suggesting that the activity of VPARP is not essential for interaction with MVP-self-assembled vault-like particles. Expand
Vaults and the major vault protein: Novel roles in signal pathway regulation and immunity
TLDR
The current knowledge on MVP and vaults is reviewed with focus on regulatory functions in intracellular signal transduction and immune defence and the definition of precise vault functions is reviewed. Expand
Nuclear localization of the major vault protein in U373 cells
TLDR
The results further support the notion that part of the cellular MVP can enter the nucleus, and that about 5% of the MVP in U373 cells is localized inside the nucleus. Expand
Expression of the Major Vault Protein (MVP) and Cellular Vault Particles in Fish
TLDR
It is shown that vaults are distributed throughout cells of fish lymphoid cells, with nuclear and plasma membrane aggregations in some cells, and MVP is highly expressed in liver neoplastic lesions in Atlantic killifish collected at the Atlantic Wood USA‐EPA superfund site on the southern branch of the Elizabeth River, VA. Expand
Movement of vault particles visualized by GFP-tagged major vault protein
TLDR
Video microscopy indicated that, whereas the majority of vault particles were stationary, some individual vault particles moved rapidly, consistent with the action of a microtubule-based or actin-based molecular motor. Expand
Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange.
TLDR
Fluorescence resonance energy transfer was observed from polyethylene glycol fused hybrid cells that expressed either CFP or YFP labeled vaults, indicating that vaults can exchange major vault protein (MVP) subunits in vivo, and suggests a means whereby vault can functionally interact with their cellular environment and deliver materials packaged within their interior. Expand
Solution structure of a two-repeat fragment of major vault protein.
TLDR
The structure of the main repeat element in human MVP, the main constituent of vaults, has a unique, novel fold that consists of a three-stranded antiparallel beta-sheet and is a starting point for understanding the self-assembly of MVP into vaults and their interactions with other proteins. Expand
Vault mobility depends in part on microtubules and vaults can be recruited to the nuclear envelope.
TLDR
Analysis of nucleo-cytoplasmic transport using a fluorescent substrate containing a classical NLS and NES expressed in MVP+/+ and MVP-/- mouse embryonic fibroblasts indicated no differences in nuclear import/export kinetics, suggesting no role for vaults in these processes. Expand
Evolution of Vault RNAs
TLDR
Vault ribonucleic acids can be traced evolutionarily along with the vault particle and its key protein components, most notably MVP, and exhibits a concserved pan-handle secondary structure and expression patterns that are often mistaken as a microRNA. Expand
Evolution of vault RNAs.
TLDR
A systematic and comprehensive analysis of this rapidly evolving class of ncRNAs in deuterostomes is reported, providing a comprehensive collection of computationally predicted vtRNA genes. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 80 REFERENCES
Evidence that vault ribonucleoprotein particles localize to the nuclear pore complex.
TLDR
The present study demonstrates that vaults specifically associate with nuclei by both immunoblotting and immunofluorescence, and confirmed that vault associate with the nuclear envelope in tissue sections and with NPCs of isolated nuclei. Expand
Vaults: Large cytoplasmic RNP's that associate with cytoskeletal elements
TLDR
Electron microscopy reveals that vaults purified from these diverse species are similar both in their dimensions and morphology (Fig. 1). Expand
Vaults. II. Ribonucleoprotein structures are highly conserved among higher and lower eukaryotes
TLDR
The isolation of vaults from numerous species is reported and it is shown that vaults appear to be ubiquitous among eukaryotes, including mammals, amphibians and Xenopus laevis, avians, and the lower Eukaryote Dictyostelium discoideum. Expand
Assembly of Vault-like Particles in Insect Cells Expressing Only the Major Vault Protein*
TLDR
These particles are the first example of a single protein polymerizing into a non-spherically, non-cylindrically symmetrical structure and will enable us to design agents that disrupt vault formation and aid in elucidating vault functionin vivo. Expand
Vaults are the answer, what is the question?
TLDR
The molecular characterization of vault components is discussed and genetic studies carried out in Dictyostelium are described, indicating a potential role for vaults in resistance of tumour cells to anticancer drugs. Expand
RNA location and modeling of a WD40 repeat domain within the vault.
TLDR
To locate the position of the vRNA, vaults were treated with RNases, and cryo-electron microscopy was performed on the resulting complexes, revealing the v RNA to be at the ends of the vault caps. Expand
Structure of the vault, a ubiquitous celular component.
TLDR
The structure of the vault is consistent with either a role in subcellular transport, as previously suggested, or in sequestering macromolecular assemblies. Expand
Characterization of the sea urchin major vault protein: a possible role for vault ribonucleoprotein particles in nucleocytoplasmic transport.
TLDR
The major vault protein in sea urchins is identified as a 107-kDa polypeptide that copurifies with microtubules and ribosomes that is present throughout the cytoplasm in eggs and embryos and in the nucleus in adult somatic cells. Expand
Vaults. III. Vault ribonucleoprotein particles open into flower-like structures with octagonal symmetry
TLDR
A structural model is proposed that predicts the stoichiometry of vault proteins and RNA, defines vault dimer-monomer interactions, and describes two possible modes for unfolding of vaults into flowers, likely to play a role in vault function. Expand
Multiple Human Vault RNAs
TLDR
The findings suggest that vaults bind the RNA molecules with different affinities in different situations, and the ratio in which the vault RNAs are associated with vaults might be of functional importance. Expand
...
1
2
3
4
5
...