The variation in frequency with which markers are transduced by phage P1 is primarily a result of discrimination during recombination

@article{Newman1980TheVI,
  title={The variation in frequency with which markers are transduced by phage P1 is primarily a result of discrimination during recombination},
  author={Barbara J. Newman and Millicent Masters},
  journal={Molecular and General Genetics MGG},
  year={1980},
  volume={180},
  pages={585-589}
}
The efficiency of recovery of P1 transductants is marker dependent and normally varies over a 25-fold range. UV irradiation of either transducing lysates or recipient cells results in a selective stimulation of the transduction of markers which are normally transduced poorly. As a result the range in frequency of transduction is reduced to about 3-fold and resembles the gene frequency distribution expected in the donor cells. We conclude that P1 transducing lysates are likely to contain a… CONTINUE READING