The value of micro-ELISA test in the diagnosis of Fasciola hepatica infection.

Abstract

OBJECTIVE In sero-diagnosis of parasitic infection, it is essential to inspect cross-reactivity between the target parasite and other parasites in order to assess diagnostic performance. The aim of this study was to determine the cut-off value of antibody titer for diagnosis of F. hepatica (FH) infection by using the micro-ELISA and diagnostic performance of this test. METHODS The study population consisted of the following groups: FH group (n=42), Echinococcus granulosus (EG) group (n=27) and control group (n=33). The micro-ELISA test for detection of anti-F. hepatica antibody was performed in all groups. RESULTS The test was positive in all patients with FH, in 3 out of 27 (11%) patients with EG and in none of the control group. Mean antibody titer was significantly higher in the FH group compared to the EG group (23.8 ± 0.9 DU vs. 5.7 ± 1.2 DU; p < 0.001) and compared to the control group (23.8 ± 0.9 DU vs. 2.4 ± 0.2 DU; p < 0.001). When we used 11,5 DU as a cut-off value for sero-diagnosis of FH, the positive predictive value was 93.3%, negative predictive value was 100%, sensitivity was 100%, and specificity was 95%. CONCLUSION Cross-reactions are an important issue in serological diagnosis of parasitic infections. The micro-ELISA test for FH antibody can not definitely discriminate fascioliasis from hydatid disease.

DOI: 10.5152/tpd.2013.06

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Cite this paper

@article{Kaya2013TheVO, title={The value of micro-ELISA test in the diagnosis of Fasciola hepatica infection.}, author={Muhsin Kaya and Remzi Beştaş and Muttalip Çiçek and Akın {\"{O}nder and Mehmet Kaplan}, journal={Turkiye parazitolojii dergisi}, year={2013}, volume={37 1}, pages={23-7} }