The vacuolar compartment is required for sulfur amino acid homeostasis inSaccharomyces cerevisiae

@article{JacqueminFaure1994TheVC,
  title={The vacuolar compartment is required for sulfur amino acid homeostasis inSaccharomyces cerevisiae},
  author={Ir{\`e}ne Jacquemin-Faure and Dominique Thomas and J. M. Laporte and Christian Cibert and Yolande Surdin-Kerjan},
  journal={Molecular and General Genetics MGG},
  year={1994},
  volume={244},
  pages={519-529}
}
In order to isolate new mutations impairing transcriptional regulation of sulfur metabolism inSaccharomyces cerevisiae, we used a potent genetic screen based on a gene fusion expressing XylE (fromPseudomonas putida) under the control of the promoter region ofMET25. This selection yielded strains mutated in various different genes. We describe in this paper the properties of one of them,MET27. Mutation or disruption ofMET27 leads to a methionine requirement and affects S-adenosylmethionine… CONTINUE READING