The utilization of 3-mercapto-2-methylpropionate as sulphur source by a phototrophic bacterium.

Abstract

A bacterium (strain photoB) photoassimilated 3-mercapto-2-methylpropionate as sole source of sulphur with methacrylate accumulating in the medium. This was thought to be the product of a sulphur-lyase type enzyme attacking the 3-mercapto-2-methylpropionate. Detailed examination of the biochemistry of the utilization of 3-mercapto-2-methylpropionate showed however that the thiol was first activated to 3-mercapto-2-methylpropionyl CoA. It appeared that this CoA-derivative then served as the substrate for a sulphur-lyase type enzyme, with methacrylyl-CoA as product. Further metabolism was via beta-hydroxyisobutyryl-CoA and beta-hydroxyisobutyrate.

Cite this paper

@article{Smith1994TheUO, title={The utilization of 3-mercapto-2-methylpropionate as sulphur source by a phototrophic bacterium.}, author={Mark R. Smith and W J van den Tweel and Jan A. M. de Bont}, journal={Bioorganic & medicinal chemistry}, year={1994}, volume={2 7}, pages={589-93} }