The use of two fluorescent dyes to identify sperm in a competitive binding assay to oocytes.

@article{Miller1998TheUO,
  title={The use of two fluorescent dyes to identify sperm in a competitive binding assay to oocytes.},
  author={D. Miller and J. M. Demers and A. Braundmeier and M. L. Behrens},
  journal={Journal of andrology},
  year={1998},
  volume={19 6},
  pages={
          650-6
        }
}
The relationship of most sperm laboratory assays to male fertility is inconsistent. Assays that measure traits required to fertilize oocytes are expected to have the most predictive value. A new assay that measures the competitive ability of two sperm samples to bind to oocytes was developed. Two populations of sperm were labeled using a pair of lipophilic dyes. A concentration of 75 microM of the two dyes, DiQ (4-[4-(dihexadecylamino)styryl]-N-methylquinolinium iodide; an orange-red dye) and… Expand
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It is concluded that the fluorescent dyes FITC and TRITC do not impair the function of human spermatozoa as assessed by motility characteristics and by their ability to penetrate ova in vitro. Expand
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Whether in vitro penetration of bovine and zona-free hamster oocytes, using spermatozoa treated with the lipid dilauroylphosphatidylcholine, was correlated with the fertility of bulls was correlated and whether utilizing results from several laboratory assays could effectively evaluate fertility was determined. Expand
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Mouse sperm Gal-transferase specifically recognizes those oligosaccharides on ZP3 that have sperm-binding activity, but does not interact with other zona pellucida glycoproteins, demonstrating a more stringent substrate specificity for the sperm enzyme. Expand
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