The use of two fluorescent dyes to identify sperm in a competitive binding assay to oocytes.

@article{Miller1998TheUO,
  title={The use of two fluorescent dyes to identify sperm in a competitive binding assay to oocytes.},
  author={D J Miller and J Marc J Demers and Andrea G. Braundmeier and Melinda L Behrens},
  journal={Journal of andrology},
  year={1998},
  volume={19 6},
  pages={650-6}
}
The relationship of most sperm laboratory assays to male fertility is inconsistent. Assays that measure traits required to fertilize oocytes are expected to have the most predictive value. A new assay that measures the competitive ability of two sperm samples to bind to oocytes was developed. Two populations of sperm were labeled using a pair of lipophilic dyes. A concentration of 75 microM of the two dyes, DiQ (4-[4-(dihexadecylamino)styryl]-N-methylquinolinium iodide; an orange-red dye) and… CONTINUE READING