The use of tryptophan mutants in identifying the 296 nm transient absorbing species during the photocycle of bacteriorhodopsin.

@article{Wu1991TheUO,
  title={The use of tryptophan mutants in identifying the 296 nm transient absorbing species during the photocycle of bacteriorhodopsin.},
  author={S G Wu and Dae Jong Jang and Mostafa A El-Sayed and Thomas Marti and Tatsushi Mogi and H Gobind Khorana},
  journal={FEBS letters},
  year={1991},
  volume={284 1},
  pages={9-14}
}
The transient absorption at 296 nm was part of the spectroscopic evidence that initiated the proposal that tyrosinate (Tyr-) is formed during, and important to, the photocycle of bacteriorhodopsin (bR). Recent evidence against such a proposal comes from the results of NMR, UV Raman as well as electron cryo-microscopic structural studies. This makes it… CONTINUE READING