The use of thin acrylamide gels for DNA sequencing

  title={The use of thin acrylamide gels for DNA sequencing},
  author={Frederick Sanger and A. R. Coulson},
  journal={FEBS Letters},
Improvements of DNA sequencing gels.
Denaturating Electrophoresis in DNA Sequencing: A Brief History and Current Protocols
Two common DNA sequencing methods rely on the principle of measuring relative distances of individual nucleotides from a fixed point in a DNA chain to determine a sequence, and a separation system which is capable of resolving two DNA fragments that differ in length by a single nucleotide is needed.
The Determination of DNA Sequences
Unlike the determination of the amino acid sequence of proteins and peptides, DNA sequence analysis is based on high-resolution electrophoresis on denaturing polyacrylamide gels of oligonucleotides with one common end, and varying in length by a single nucleotide at the other end.
Electrophoresis and Blotting of DNA
Despite the introduction of more powerful methods such as polymerase chain reaction and DNA sequencing, electrophoresis and blotting techniques are still widely used to identify DNA fragments of interest, including the identification of unusual structures within DNA and the analysis of larger fragments which are not easily analysed by other methods.
Microelectrophoresis for the separation of DNA fragments
Analysis of the microgels with proteins of known size indicates that separations are occurring in gels with pore sizes close to the diameter of double‐stranded DNA, which indicates that DNA sequencing may be possible with further optimization.
Field gradients improve resolution on DNA sequencing gels.


A new method for sequencing DNA.
  • A. Maxam, W. Gilbert
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1977
Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Nucleotide sequence of bacteriophage φX174 DNA
The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.