942 Citations
Elimination of the secondary structure effect in gel sequencing of nucleic acids
- Biology, ChemistryFEBS letters
- 1980
Denaturating Electrophoresis in DNA Sequencing: A Brief History and Current Protocols
- Biology
- 1998
Two common DNA sequencing methods rely on the principle of measuring relative distances of individual nucleotides from a fixed point in a DNA chain to determine a sequence, and a separation system which is capable of resolving two DNA fragments that differ in length by a single nucleotide is needed.
The Determination of DNA Sequences
- Biology, Chemistry
- 1983
Unlike the determination of the amino acid sequence of proteins and peptides, DNA sequence analysis is based on high-resolution electrophoresis on denaturing polyacrylamide gels of oligonucleotides with one common end, and varying in length by a single nucleotide at the other end.
Electrophoresis and Blotting of DNA
- Biology, Chemistry
- 2013
Despite the introduction of more powerful methods such as polymerase chain reaction and DNA sequencing, electrophoresis and blotting techniques are still widely used to identify DNA fragments of interest, including the identification of unusual structures within DNA and the analysis of larger fragments which are not easily analysed by other methods.
Direct sequence and footprint analysis of yeast DNA by primer extension.
- BiologyMethods in enzymology
- 1991
Microelectrophoresis for the separation of DNA fragments
- Chemistry, BiologyElectrophoresis
- 1992
Analysis of the microgels with proteins of known size indicates that separations are occurring in gels with pore sizes close to the diameter of double‐stranded DNA, which indicates that DNA sequencing may be possible with further optimization.
DNA sequencing with direct transfer electrophoresis and nonradioactive detection.
- BiologyMethods in enzymology
- 1993
Field gradients improve resolution on DNA sequencing gels.
- BiologyJournal of biochemical and biophysical methods
- 1984
References
SHOWING 1-6 OF 6 REFERENCES
A new method for sequencing DNA.
- Biology, ChemistryProceedings of the National Academy of Sciences of the United States of America
- 1977
Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
- Biology, ChemistryJournal of molecular biology
- 1975
A simple apparatus for vertical flat-sheet polyacrylamide gel electrophoresis.
- PhysicsAnalytical biochemistry
- 1968
Nucleotide sequence of the intercistronic region between genes G and F in bacteriophage phiX174 DNA.
- Biology, ChemistryJournal of molecular biology
- 1976
Nucleotide sequence of bacteriophage φX174 DNA
- BiologyNature
- 1977
The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.