The use of the phosphomannose isomerase gene as alternative selectable marker for Agrobacterium-mediated transformation of flax (Linum usitatissimum)

@article{Lamblin2006TheUO,
  title={The use of the phosphomannose isomerase gene as alternative selectable marker for Agrobacterium-mediated transformation of flax (Linum usitatissimum)},
  author={Fr{\'e}d{\'e}ric Lamblin and Aur{\'e}lie Aim{\'e} and Christophe Hano and Isabelle Roussy and Jean-Marc Domon and Bart Van Droogenbroeck and {\'E}ric Lain{\'e}},
  journal={Plant Cell Reports},
  year={2006},
  volume={26},
  pages={765-772}
}
In order to meet the future requirement of using non-antibiotic resistance genes for the production of transgenic plants, we have adapted the selectable marker system PMI/mannose to be used in Agrobacterium-mediated transformation of flax (Linum usitatissimum L.) cv. Barbara. The Escherichia coli pmi gene encodes a phosphomannose isomerase (E.C. 5.1.3.8) that converts mannose-6-phosphate, an inhibitor of glycolysis, into fructose-6-phosphate (glycolysis intermediate). Its expression in… CONTINUE READING
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