The use of the phosphomannose-isomerase/mannose selection system to recover transgenic apple plants

@article{Degenhardt2006TheUO,
  title={The use of the phosphomannose-isomerase/mannose selection system to recover transgenic apple plants},
  author={Juliana Degenhardt and Annika Poppe and Jurith Montag and Iris Szankowski},
  journal={Plant Cell Reports},
  year={2006},
  volume={25},
  pages={1149-1156}
}
A selection system based on the phosphomannose-isomerase gene (pmi) as a selectable marker and mannose as the selective agent was evaluated for the transformation of apple (Malus domestica Borkh.). Mannose is an unusable carbon source for many plant species. After uptake, mannose is phosphorylated by endogenous hexokinases to mannose-6-phosphate. The accumulation of mannose-6-phosphate leads to a block in glycolysis by inhibition of phosphoglucose-isomerase, resulting in severe growth… CONTINUE READING
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