The use of the modified hypo-osmotic swelling test for the selection of immotile testicular spermatozoa in patients treated with ICSI: a randomized controlled study.

@article{Sallam2005TheUO,
  title={The use of the modified hypo-osmotic swelling test for the selection of immotile testicular spermatozoa in patients treated with ICSI: a randomized controlled study.},
  author={Hassan N. Sallam and Ashraf Farrag and Abdel Fattah Agameya and Yehia F Elgarem and Fathy Ezzeldin},
  journal={Human reproduction},
  year={2005},
  volume={20 12},
  pages={
          3435-40
        }
}
BACKGROUND Various methods for the selection of viable sperm from among immotile testicular spermatozoa for use in ICSI have been described in non-randomized studies. We have conducted a randomized controlled study to compare the use of the modified hypo-osmotic swelling (HOS) test (50% culture medium + 50% Milli-Q grade water) with that of sperm selection on the basis of their morphology alone. METHODS A total of 79 couples with immotile testicular spermatozoa treated with ICSI were randomly… 
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In men with NOA, the ICSI outcome is not affected by whether the retrieved testicular sperm is fresh or frozen, and sperm cryopreservation ought to be considered in every surgical sperm retrieval case, which remain feasible even in patients with few testicular semen retrieved.
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The present review provides an up-to-date summary of current strategies for maximizing sperm recovery from surgically obtained testicular samples and, as an extension, optimization of in vitro sperm processing techniques in the management of NOA.
Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes
TLDR
The latest techniques for the preparation and selection of human spermatozoa are discussed focusing on those proven to improve one or several of the following parameters: sperm genetic integrity, fertilization capacity, embryo production, and in vitro survival, as well as pregnancy and delivery rates following in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).
Optimizing TESE‐ICSI by laser‐assisted selection of immotile spermatozoa and polarization microscopy for selection of oocytes
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The fertilization rate with immotile, but vital spermatozoa and the use of polarization microscopy can enhance the FR of TESE‐ICSI, which clearly benefits from laser selection and is a non‐hazardous and safe method for the selection of viable but immotiles sperm.
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