The use of the mechanical fragility test in evaluating sublethal RBC injury during storage

  title={The use of the mechanical fragility test in evaluating sublethal RBC injury during storage},
  author={Jitesh S Raval and Jonathan H. Waters and Axel Seltsam and Erwin A. Scharberg and E Richter and Amanda R Daly and Marina V. Kameneva and Mark H. Yazer},
  journal={Vox Sanguinis},
Background  The mechanical fragility index (MFI) is an in vitro measurement of the extent of RBC sublethal injury. Sublethal injury might constitute a component of the RBC storage lesion, thus the MFI was determined serially during routine RBC storage. 

Haemolysis and sublethal injury of RBCs after routine blood bank manipulations

Objectives: To determine the extent of RBC sublethal injury in male donor units as measured by both the mechanical fragility index (MFI) and percentage haemolysis after RBCs underwent leucoreduction

The impact of suctioning RBCs from a simulated operative site on mechanical fragility and hemolysis

During static storage pre- menopausal female RBCs in CPD-preserved whole blood demonstrated higher shear stress tolerance compared to the males or post-menopausal females at all of the time points, but enhanced resistance was not observed after suctioning from a simulated surgical field.

The effect of stationary versus rocked storage of whole blood on red blood cell damage and platelet function

Increasingly, cold‐stored whole blood (WB) is being considered for the resuscitation of civilian trauma patients. It is unclear whether the WB should be agitated to enhance the function of the

RBC mechanical fragility as a direct blood quality metric to supplement storage time.

Findings indicate that MF profiles can provide a powerful and versatile tool for investigation of RBC, as well as a potential metric of R BC quality.

Red Blood Cell Mechanical Fragility Test for Clinical Research Applications

The new reduced volume RBT and modified MFI will allow for the measurement of RBC MF in clinical and preclinical studies involving humans or small animals.

Mechanical Fragility as a Potential Time-Independent Measure of Membrane Integrity among Stored RBC Units

RBC membrane properties, as defined by MF, vary markedly across RBC units, and in some, within the same units which resulted in distinguishable subpopulations.

Red blood cell mechanical stability test.

The Couette type ektacytometer can be used as a tool to assess the sub-hemolytic damage to RBC in testing the biomedical equipment and estimate the hemolytic threshold as a measure of RBC mechanical stability.

Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

The findings of the present and preceding studies suggest that different St-RBC properties are differentially reversed by RJ, implying that some of the changes occur during storage and are irreversible.

In vitro quality and hemostatic function of cold‐stored CPDA‐1 whole blood after repeated transient exposure to 28°C storage temperature

Blood products are frequently exposed to room temperature or higher for longer periods than permitted by policy. We aimed to investigate if this resulted in a measurable effect on common quality

An approach to measuring RBC haemolysis and profiling RBC mechanical fragility

This article reports on a unique approach to measuring haemolysis, without the need for centrifugation or other sample separation, utilising an electromagnet to cause a bead to oscillate within a cartridge that contains the sample.



A comparison of hemolysis and red cell mechanical fragility in blood collected with different cell salvage suction devices

This study compared a constant‐suction‐pressure system with a device that is expected to minimize hemolysis by automatically varying the suction pressure.

Influence of storage on red blood cell rheological properties.

Serious hemorrheological disorders, including the decrease in RBC deformability secondary to shape abnormalities, acidosis, and the decrease of blood clotting, start already at the second week of storage and progress up to the end of the storage period.

Alteration of red cell aggregability and shape during blood storage

Storage of blood units has been reported to induce changes in red cells that are expected to alter their aggregability, and this study aims to evaluate the effects of storage time on red cells' aggregability.

Mechanical Fragility Calibration of Red Blood Cells

Five mechanical fragility test systems are compared and all seem to measure the same parameter; the hemoresistometer most closely matched the pump test results, but the stainless steel bead test may be the most practical for routine calibration purposes.

Effect of perfluorochemical emulsion on blood trauma and hemorheology.

Results of this study demonstrate the potential feasibility of Fluosol to improve mechanical properties of blood in patients with heart assist devices and reduce mechanical fragility of human and ovine red blood cells.

Transfusion of stored red blood cells adhere in the rat microvasculature

This study determined whether duration of RBC storage and LR influence RBC adhesion in vivo in capillaries and showed that human RBCs adhere to endothelial monolayers in vitro with prolonged storage and is reduced by prestorage leukoreduction.

Effect of red cell age on vesiculation in vitro

Microvesiculation of RBC occurs in vivo and during in vitro storage and quantitation of protein, cholesterol and phospholipids showed significantly larger amounts in the vesicles shed by the younger RBC than by the older RBC.

Is red blood cell rheology preserved during routine blood bank storage?

The rheologic properties and cell variables of leukoreduced RBC units, during routine blood bank storage in saline‐adenine‐glucose‐mannitol, were investigated.

The membrane and the lesions of storage in preserved red cells

AN ANECDOTE RELATED by Dr. Diamond depicts the extent of the storage lesions in banked blood. Dr. J.G. Gibson, when he was studying blood preservatives, requested a specimen for his own analysis from

Prestorage leukoreduction and low‐temperature filtration reduce hemolysis of stored red cell concentrates

A pool‐split design tested the effects of leukoreduction on hemolysis of stored RBCs and concluded that stored red cells (RBCs) are more hemolyzed than their unfiltered predecessors.