The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)

@article{Boscariol2003TheUO,
  title={The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)},
  author={R. L. Boscariol and Weliton Antonio Bastos de Almeida and M. T. V. C. Derbyshire and Francisco de Assis Alves Mour{\~a}o Filho and Beatriz Madalena Januzzi Mendes},
  journal={Plant Cell Reports},
  year={2003},
  volume={22},
  pages={122-128}
}
A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of… CONTINUE READING
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High efficiency Agrobacterium-mediated transformation and regeneration of citrus

  • L Pe a, M Cervera, +4 authors L Navarro
  • Plant Sci
  • 1995
Highly Influential
4 Excerpts

Genetic transformation of lime (Citrus aurantifolia Swing.): factors affecting transformation and regeneration

  • L Pe a, M Cervera, J Ju rez, A Navarro, JA Pina, L Navarro
  • Plant Cell Rep 16:731–737
  • 1997
Highly Influential
1 Excerpt

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