The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins.

@article{Grslund2008TheUO,
  title={The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins.},
  author={Susanne Gr{\"a}slund and Johanna Sagemark and Helena Berglund and Lars-G{\"o}ran Dahlgren and Alex Flores and Martin Hammarstr{\"o}m and Ida Johansson and Tetyana Kotenyova and Martina Nilsson and Paer Nordlund and Johan Weigelt},
  journal={Protein expression and purification},
  year={2008},
  volume={58 2},
  pages={
          210-21
        }
}
Bacterial over-expression of proteins is a powerful tool to obtain soluble protein amenable to biochemical, biophysical and/or structural characterization. However, it is well established that many recombinant proteins cannot be produced in a soluble form. Several theoretical and empirical methods to improve soluble production have been suggested, although there is to date no universally accepted protocol. This report describes, and quantitatively analyses, a systematic multi-construct approach… CONTINUE READING

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