The use of silver nitrate as a stain for scanning electron microscopy of arterial intima and paraffin sections of kidney

@article{Geissinger1972TheUO,
  title={The use of silver nitrate as a stain for scanning electron microscopy of arterial intima and paraffin sections of kidney},
  author={H. Dieter Geissinger},
  journal={Journal of Microscopy},
  year={1972},
  volume={95}
}
The suitability of silver nitrate as a stain for scanning electron microscopy was investigated. Accordingly en face preparations of arterial intima were impregnated with silver nitrate, fixed with formalin, and coated with platinum‐palladium alloy. In SEM images, the silver lines surrounding the endothelial cells, and the deposits on the intima appear as white lines or dots on a darker background. Similar results were noted for nitrocellulose‐embedded endothelium. Paraffin sections of kidney… 
15 Citations
Identification of nerve fibres in the scanning electron microscope
TLDR
The use of silver as a ‘stain’ for nerve fibres in scanning electron microscopy (SEM) preparations has been investigated and nerve fibre blocks were identified in the same tissue, but different blocks by means of transmission electron microscope studies and by fluoroescence microscopy.
Scanning Electron Microscopic observations of endothelial changes in experimentally induced atheromatosis of rabbit aortas
Following the administration of cholesterol for a period of 6–7 weeks, Scanning Electron Microscopic (S.E.M.) observations revealed mono-cellular, crater-like and dome-shaped endothelial changes on
Scanning and Transmission Electron Microscopy of the Ependymal Lining of the Third Ventricle
  • J. Bruni
  • Biology
    Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques
  • 1974
TLDR
The significance of ependymal tanycytes particularly of the third ventricle derives largely from the connections they establish between the ventricular lumen and vasculature of the median eminence, which has led to the suggestion that ependyma cells and cerebrospinal fluid of theThird ventricles may be involved in the regulation of adenohypophysial activity.
Fine structure of “intimal cushions” at branching sites in coronary arteries of vertebrates
TLDR
The branching sites of perfusion-fixed coronary arteries of Sprague-Dawley rat, zebra finch and lizard were studied by scanning and transmission electron microscopy and a peculiar type of cushion was observed in smaller arterioles, suggesting that thistype of cushion is closely associated with the precapillary sphincters.
Problems with the preparation of blood vessels for scanning electron microscopy a critical review
The application of the scanning electron microscope (SEM) in the investigation of blood vessels in the last ten years is reviewed. Methodological differences from anesthesia of the experimental
A precise stage arrangement for correlative microscopy for specimens mounted on glass slides, stubs or EM grids
The instrumentation necessary for precise and fast correlation of images derived from a light optical microscope (LM) and a scanning electron microscope (SEM) operated in the reflective mode, is
Staining of biological material for the scanning electron microscope
TLDR
A histochemical technique used in scanning electron microscopy to localize specific areas in biological material and depends on the interaction of complex variables such as the coating material, the accelerating voltage and the use of a primary or secondary electron signal.
...
...

References

SHOWING 1-10 OF 39 REFERENCES
Correlateci light optical and scanning electron microscopy of Gram smears of bacteria and paraffin sections of cardiac muscle *
TLDR
Light‐microscope slides bearing Gram smears of Erysipelothrix rhusiopathiæ, or Staphylococcus aureus, after preliminary examination under the light‐optical microscope, served as a control for investigations into bacteria‐cell junctions in tissue.
Autoradiography in the Scanning Electron Microscope
TLDR
Although the resolution of about 1000 Å in electron microscopic autoradiography with tritium is far lower than that in common electron microscopic experiments, ultrastructure research has been aided in important instances by this technique.
The occurrence of microthrombi on the aortic endothelium of swine.
Evidence is presented in this report that microthrombi composed chiefly of platelets form on what appears to be morphologically normal swine endothelium and that the pattern of the deposits
THE EXAMMINATION OF INJURED AND NORMAL AORTIC ENDOTHELIUM OF SWINE
Faculty of the Graduate School of the University of Toronto In Partial Fulfillment of the Master of Veterinary Science by Hans Dieter Geissinger October, 1962 PLEASE NOTE: Due to the blurriness of
Histopathologic Technic and Practical Histochemistry
TLDR
Histopathologies technic and practical histochemistry, Histopathologie techno-practical histochemistry and the role of nanofiltration in medicine and drug discovery and abuse are studied.
Endothelial cells and cement lines.
Histological and histochemical technics
...
...