The use of semi-automated fluorescent microsatellite analysis for tomato cultivar identification

@article{Bredemeijer1998TheUO,
  title={The use of semi-automated fluorescent microsatellite analysis for tomato cultivar identification},
  author={G. M. M. Bredemeijer and Paul Arens and Doret Wouters and Dirk Visser and Ben Vosman},
  journal={Theoretical and Applied Genetics},
  year={1998},
  volume={97},
  pages={584-590}
}
  • G. M. M. Bredemeijer, Paul Arens, +2 authors Ben Vosman
  • Published 1998
  • Biology
  • Theoretical and Applied Genetics
  • Abstract The objectives of this study were to evaluate the usefulness of a fluorescent-analysis method for genotyping PCR-based tomato microsatellite markers (or STMSs) and to establish the value of these markers to generate unique DNA profiles of tomato cultivars. The analyses were performed using forward primers labelled with a fluorochrom and using an ALF express DNA sequencer. In general, analysis of the tomato STMSs revealed distinct allelic peaks. PCR artefacts like stuttering and… CONTINUE READING

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