The use of real-time quantitative polymerase chain reaction to detect hypermethylation of the CpG islands in the promoter region flanking the GSTP1 gene to diagnose prostate carcinoma.

@article{Chu2002TheUO,
  title={The use of real-time quantitative polymerase chain reaction to detect hypermethylation of the CpG islands in the promoter region flanking the GSTP1 gene to diagnose prostate carcinoma.},
  author={D C Chu and Cheng-Keng Chuang and Jin-Bao Fu and Hsien-Siang Huang and Ching-Ping Tseng and C G Sun},
  journal={The Journal of urology},
  year={2002},
  volume={167 4},
  pages={1854-8}
}
PURPOSE We developed a real-time, quantitative, methylation sensitive polymerase chain reaction (PCR) protocol to analyze hypermethylation of the CpG islands in the promoter region of the pi class glutathione-S-transferase gene GSTP1 in prostate cancer tissue. MATERIALS AND METHODS A total of 21 prostate cancer and 72 benign prostate hyperplasia (BPH) tissue samples were analyzed. Genomic DNA was digested with restriction enzyme, followed by real-time quantitative PCR amplification. Cycle… CONTINUE READING