The use of random amplified polymorphic DNA markers in wheat

@article{Devos1992TheUO,
  title={The use of random amplified polymorphic DNA markers in wheat},
  author={Katrien M. Devos and Mike D. Gale},
  journal={Theoretical and Applied Genetics},
  year={1992},
  volume={84},
  pages={567-572}
}
An evaluation was made of the use of random amplified polymorphic DNA (RAPD) as a genetic marker system in wheat. Reproducible amplification products were obtained from varietal, homozygous single chromosome recombinant line and wheat/alien addition line genomic DNA with selected primers and rigorously optimized reaction conditions. Factors influencing the RAPD patterns are DNA concentration, Mg2+ concentration, polymerase concentration and denaturing temperature. In wheat, the non-homoeologous… CONTINUE READING
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