The use of random amplified polymorphic DNA markers in wheat

  title={The use of random amplified polymorphic DNA markers in wheat},
  author={Katrien M. Devos and Mike D. Gale},
  journal={Theoretical and Applied Genetics},
An evaluation was made of the use of random amplified polymorphic DNA (RAPD) as a genetic marker system in wheat. Reproducible amplification products were obtained from varietal, homozygous single chromosome recombinant line and wheat/alien addition line genomic DNA with selected primers and rigorously optimized reaction conditions. Factors influencing the RAPD patterns are DNA concentration, Mg2+ concentration, polymerase concentration and denaturing temperature. In wheat, the non-homoeologous… CONTINUE READING
Highly Influential
This paper has highly influenced 13 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS


Publications citing this paper.
Showing 1-10 of 176 extracted citations


Publications referenced by this paper.
Showing 1-10 of 12 references

RAPD markersa new technology for genetic mapping and plant breeding

  • ER Sears
  • AgBiotech News Inf
  • 1991

Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by random amplified polymorphic DNA assay

  • Islam AKMR, KW Shepherd, Sparrow DHB
  • Appl Environ Microbiol
  • 1991

Location of tamylase sequences in wheat and its relatives

  • S Weining, P Langridge
  • 1988

Isolation and addition of euplasmic wheatbarley addition lines

  • N Maia
  • Heredity
  • 1981

A synthetic hexaploid wheat with fragile rachis

  • PJ Sharp, M Kreis, PR Shewry, Gale
  • Wheat Inf Serv
  • 1976

Similar Papers

Loading similar papers…