The use of pronase-digested human leukocytes to improve specificity of the flow cytometric crossmatch.

@article{Lobo1995TheUO,
  title={The use of pronase-digested human leukocytes to improve specificity of the flow cytometric crossmatch.},
  author={Peter Isaac Lobo and Clint E Spencer and William C. Stevenson and C Mccullough and Timothy L. Pruett},
  journal={Transplant international : official journal of the European Society for Organ Transplantation},
  year={1995},
  volume={8 6},
  pages={472-80}
}
Two-color fluorescence cytometry (FCXM) has recently been introduced to improve the detection of anti-HLA antibodies that react to donor cells, especially in recipients receiving kidney allografts. Although this assay system is highly sensitive, it lacks specificity. Between 70% and 90% of potential kidney recipients with a positive FCXM would have been denied transplant if such an assay had been used alone to detect antidonor antibodies. Lack of specificity is principally due to normal or… CONTINUE READING