The use of planarians to dissect the molecular basis of metazoan regeneration

@article{Alvarado1998TheUO,
  title={The use of planarians to dissect the molecular basis of metazoan regeneration},
  author={A. S. Alvarado and P. Newmark},
  journal={Wound Repair and Regeneration},
  year={1998},
  volume={6}
}
Freshwater planarians possess remarkable regenerative abilities that make them one of the classic model organisms for the study of regeneration. These free‐living members of the phylum Platyhelminthes are representatives of the simplest triploblastic organisms possessing bilateral symmetry and cephalization. Furthermore, planarians occupy an important position in the evolution of Metazoa, which allows for the possibility of vertically integrating molecular studies of regeneration in this… Expand
The Case for Comparative Regeneration: Learning from Simpler Organisms How to Make New Parts from Old
TLDR
It is argued for the reinstatement of planarians, a classical and currently understudied experimental organism, as a viable, molecular model system in which to functionally dissect the molecular basis of animal regeneration. Expand
The Schmidtea mediterranea database as a molecular resource for studying platyhelminthes, stem cells and regeneration
TLDR
The characterization of ∼3000 non-redundant cDNAs from a clonal line of the planarian Schmidtea mediterranea, and the ability of abrogating gene expression in planarians using RNA interference technology, pave the way for a systematic study of the remarkable biological properties displayed by Platyhelminthes. Expand
Double-stranded RNA specifically disrupts gene expression during planarian regeneration.
TLDR
The ability to eliminate gene function in a regenerating organism such as the planarian overcomes previous experimental limitations and opens the study of animal regeneration to unprecedented levels of molecular detail. Expand
Advancing the flatworm Macrostomum lignano as a versatile model organism for stem cell research
TLDR
This study suggests conserved functions of DDX39 among species and convenience of using Macrostomum lignano as a complementary model to mammals and shows the regulatory roles of Hippo pathway in proliferation and selfrenewal of neoblasts. Expand
Planarians: an In Vivo Model for Regenerative Medicine
TLDR
Free-living freshwater hermaphrodite Schmidtea mediterranea has emerged as a suitable model system because it displays robust regenerative properties and, unlike most other planarians, it is a stable diploid with a genome size nearly half that of other common planarians. Expand
Characterization of innexin gene expression and functional roles of gap-junctional communication in planarian regeneration.
TLDR
The expression data and the respecification of the posterior blastema to an anteriorized fate by GJC loss-of-function suggest that innexin-based GJC mediates instructive signaling during regeneration. Expand
Fundamentals of planarian regeneration.
TLDR
The types of structures produced by blastemas on a variety of wound surfaces, the principles guiding the reorganization of pre-existing tissues, and the manner in which scale and cell number proportions between body regions are restored during regeneration are identified. Expand
Regeneration in the metazoans: why does it happen?
TLDR
This essay will review the distribution and the modes of regeneration that are found in the different metazoan phyla, and re-examine ideas on its evolutionary origins, and discuss its possible relationship to both asexual reproduction and embryogenesis. Expand
Molecular and Cellular Basis of Regeneration and Tissue Repair
TLDR
First steps have been undertaken to understand how neoblast and differentiated cells communicate with each other to adapt the self-renewal and differentiation rates of neoblasts to the demands of the body. Expand
Cytoskeletal proteins and morphogenesis in planarians
TLDR
Results concerning two cytoskeleton components, actin and tubulin, during morphogenetic processes of the planarian Schmidtea polychroa are discussed. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 38 REFERENCES
Planarian Hox genes: novel patterns of expression during regeneration.
TLDR
Sequence analyses of seven planarian Dthox genes reveal high similarities with the homeodomain region of the Hox cluster genes, allowing us to assign planarian Hox-related genes to anterior and medial Hox clusters paralogous groups. Expand
Identification of members of several homeobox genes in a planarian using a ligation-mediated polymerase chain reaction technique.
TLDR
The hypothesis that the tremendous diversity of metazoan body plans is specified by a largely conserved array of homeobox-containing developmental genes is supported. Expand
Growth, Degrowth and Regeneration as Developmental Phenomena in Adult Freshwater Planarians
TLDR
Freshwater planarians are the best known planarians due to there easy culture and ease of handling under laboratory conditions and, because they have been, and still are, the most widely used turbellarian in experimental research, particularly with regards to regeneration. Expand
Recuperation of regeneration in denervated limbs of Ambystoma larvae
The current neurotrophic theory of amphibian limb regeneration allows for a non-neural tissue contribution of trophic substance (TS) which during ontogeny is suppressed by the production of largeExpand
A central body region defined by a position-specific molecule in the planarian Dugesia (Girardia) tigrina: spatial and temporal variations during regeneration.
TLDR
Immunogold light and electron microscopy data suggest that expression of TCEN49 antigen is very sensitive to positional information changes and that it could be involved in the expression of central-body positional identity. Expand
Regulation of HoxA expression in developing and regenerating axolotl limbs.
TLDR
The HoxA complex, which is thought to be involved in pattern formation along the proximal-distal limb axis, is reported on, with reports on the expression patterns of two 5' members of this complex, H CoxA13 and Hox a9. Expand
The Elements of Experimental Embryology
THIS work is a rather surprising addition to the Cambridge Comparative Physiology series—surprising partly because it is twice as heavy as any of the earlier members of the series, and partly becauseExpand
The effects of denervation on the ultrastructure of young limb regenerates in the newt, Triturus.
TLDR
It is proposed that the failure of regeneration following denervation is due to a relative decrease in number and possible transformation of many of these cells. Expand
Neurotrophic activity of brain extracts in forelimb regeneration of the urodele, Triturus.
The loss in protein synthesis which the regenerating forelimb of the newt suffers after denervation can be recovered by infusing into it an extract of newt soluble brain protein. Moreover, theExpand
The nerve fiber content of Amblystoma aneurogenic limbs.
TLDR
The nerveless state of larval Amblystoma limbs rendered aneurogenic by removal of the embryonic neural tube and crest was examined and the number of fibers was less than one-tenth of that in the normal limb and thus well below the expected level required for regeneration. Expand
...
1
2
3
4
...