The use of non-aqueous chloroform/methanol extraction for the delipidation of brain with minimal loss of enzyme activities.

Abstract

When freeze-dried brain was extracted at -4-0degrees C with dry chloroform/methanol (2:1 v/v), four of the five enzymes examined were recovered in the diethyl ether-washed residue without inactivation. By contrast, extraction with chloroform/methanol (2:1 v/v) in the presence of water destroyed activities of all the enzymes examined. The amounts of major… (More)

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Cite this paper

@article{Kurihara1977TheUO, title={The use of non-aqueous chloroform/methanol extraction for the delipidation of brain with minimal loss of enzyme activities.}, author={Takeshi Kurihara and Yoshiki Nishizawa and Y. Takahashi}, journal={The Biochemical journal}, year={1977}, volume={165 1}, pages={135-40} }