The use of microsatellite analysis in population biology: background, methods and potential applications.

  title={The use of microsatellite analysis in population biology: background, methods and potential applications.},
  author={Mary V. Ashley and Beverly D. Dow},
A promising technique currently emerging for population studies is the analysis of microsatellite DNAs. Microsatellite alleles offer several advantages over other types of molecular markers. They are abundant, highly variable, and can be assayed from minute quantities of DNA using PCR. Their major disadvantage is that it is usually necessary to screen genomic libraries to identify and characterize microsatellite loci for each species under investigation. The first goal of this chapter is to… 
High-utility conserved avian microsatellite markers enable parentage and population studies across a wide range of species
This new set of conserved markers will not only reduce the necessity and expense of microsatellite isolation for a wide range of genetic studies, including avian parentage and population analyses, but will also now enable comparisons of genetic diversity among different species (and populations) at the same set of loci, with no or reduced bias.
Molecular Markers, Gene Flow, and Natural Selection
The utility of various molecular markers for addressing microevolutionary questions is discussed, focusing on recently developed DNA markers rather than on isozymes, because some readers may be less familiar with DNA methods than they are with protein electrophoresis.
Novel microsatellite markers for the analysis of Phytophthora infestans populations
Co-dominant microsatellite molecular markers for Phytophthora infestans were developed and their potential for monitoring the genetic variation in populations was demonstrated in the UK, across
Challenges of microsatellite isolation in fungi.
DNA Analyses in Avian Ecological Studies
The outline of Sibley's tapestry, examples of studies utilizing the three approaches of molecular phylogeny, molecular genetics and conservation genetics are described and molecular tools, ethical problems and future possibilties are discussed.
Characterization of new Schistosoma mansoni microsatellite loci in sequences obtained from public DNA databases and microsatellite enriched genomic libraries.
Microsatellites were present in 67 out of 300 sequences from microsatellite enriched libraries (55 perfect, 38 imperfect and 15 compounds) and 55 were selected for having the longest perfect repeats and flanking regions that allowed the design of primers for PCR amplification.
Microsatellite markers – a tool for molecular characterization of cattle genetic resources
This review gives a brief summary on the application of one of more advanced DNA-based molecular markers in cattle breeding – the microsatellites.
Insect Population Ecology and Molecular Genetics
  • M. Hoy
  • Biology
    Insect Molecular Genetics
  • 2019


A new strategy useful for rapid identification of microsatellites from DNA libraries with large size inserts.
A new strategy, based both on the use of a microsatellite specific probing and on the creation of nested deleted clones with the Exonuclease III, is described in order to position microsatellites in a range allowing direct sequencing.
Detection of highly polymorphic microsatellite loci in a species with little allozyme polymorphism
This method demonstrates that trinucleotide microsatellite loci are abundant and highly polymorphic in the social wasp Polistes annularis, whereas allozyme electrophoresis reveals very little polymorphism.
PCR-amplified microsatellites as markers in plant genetics.
Polymerase chain reaction amplification of ( AT)n and (TAT)n microsatellites in soybean revealed that they are highly polymorphic, as a consequence of length variation, somatically stable and inherited in a co-dominant Mendelian manner.
Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites.
Fifty sequences from the mouse genome database containing simple sequence repeats or microsatellites have been analysed for size variation using the polymerase chain reaction and gel electrophoresis to facilitate construction of high resolution maps of both the mouse and human genomes.
Mitochondrial DNA in natural populations: An improved routine for the screening of genetic variation based on sensitive silver staining
This report describes a combination of laboratory techniques – isolation of mtDNA by either density ultracentrifugation or a phenol extraction procedure and DNA fragment visualization by a sensitive silver staining technique that removes the major restriction of tissue sample size and number of individuals that can be screened.
The synthetic (TG)n polydinucleotide: a probe for gene flow and paternity studies in wild plant populations?
Alternative techniques, potentially capable of detecting fingerprint levels of variability and suitable for gene flow and paternity studies in wild plant populations, are currently being sought.
The abundance of various polymorphic microsatellite motifs differs between plants and vertebrates.
The abundance of different simple sequence motifs in plants was accessed through data base searches of DNA sequences and quantitative hybridization with synthetic dinucleotide repeats and the GT/CA motif being the most abundant din nucleotide repeat in mammals was found to be considerably less frequent in plants.
Polymerase-Chain-Reaction (PCR) Analysis of Microsatellites: A New Approach to Studies of Genetic Relationships in Birds
The occurrence of nonparental alleles in offspring from two Barn Swallow families was consistent with extrapair fertilization as revealed by a parallel DNAfingerprinting analysis.
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.
A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.