The use of microfluorometric method for activity-guided isolation of antiplasmodial compound from plant extracts

  title={The use of microfluorometric method for activity-guided isolation of antiplasmodial compound from plant extracts},
  author={Mohammed Nasir Shuaibu and Ponchang Apollos Wuyep and Tetsuo Yanagi and Kenji Hirayama and T. Tanaka and Isao Kouno},
  journal={Parasitology Research},
In vitro antiplasmodial activity of methanolic extracts of 16 medicinal plants was evaluated by fluorometric assay using PicoGreen. The IC50s, as determined by parasite DNA concentration, ranged from <11 to >200 and <13 to >200 μg/ml for Plasmodium falciparum 3D7 and K1, respectively; and the most active extracts were those from Anogeissus leiocarpus and Terminalia avicennoides (<11–≥14 μg/ml). Aqueous, butanolic, ethyl acetate, and methanolic fractions of these two extracts revealed butanolic… 

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