The use of microRNAs as reference genes for quantitative polymerase chain reaction in soybean.

@article{Kulcheski2010TheUO,
  title={The use of microRNAs as reference genes for quantitative polymerase chain reaction in soybean.},
  author={Franceli Rodrigues Kulcheski and Francismar Correa Marcelino-Guimar{\~a}es and Alexandre Lima Nepomuceno and Ricardo Vilela Abdelnoor and Rogerio Margis},
  journal={Analytical biochemistry},
  year={2010},
  volume={406 2},
  pages={185-92}
}
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a robust and widely applied technique used to investigate gene expression. However, for correct analysis and interpretation of results, the choice of a suitable gene to use as an internal control is a crucial factor. These genes, such as housekeeping genes, should have a constant expression level in different tissues and across different conditions. The advances in genome sequencing have provided high-throughput… CONTINUE READING

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