The use of lectins in histopathology

@article{Walker1989TheUO,
  title={The use of lectins in histopathology},
  author={Rosemary A. Walker},
  journal={Histopathology},
  year={1989},
  volume={9}
}
  • R. Walker
  • Published 1 October 1985
  • Biology, Chemistry
  • Histopathology
Lectins are proteins and glycoproteins extracted predominantly from plants which have the capacity to bind sugars specifically. This property makes them of interest for histopathology since they will bind to saccharides forming parts of glycoproteins and glycolipids of tissue constituents. Lectins have and can be used as reagents for mucin histochemistry, to identify specific cells, in the recognition of glycoprotein alterations in disease states, in studies of infectious diseases, and in the… 
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The present lectin histochemical study revealed a distinctive pattern of oligosaccharide distribution in the lungs and placenta of B. abortus-infected fetuses.
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The present study shows that these lectins have limited value in the elucidation of oral carcinogenesis and are of insignificant diagnostic value.
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Pancreatic carcinomas and chronic pancreatitis bound all five lectins without any qualitative distinction from each other or from normal pancreatic tissue, but there was increased intensity of peanut agglutinin binding to secreted mucins in pancreatic carcinoma, which may be of potential use in radiolabelled lectin scanning.
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It seems reasonable to assume that the lectin‐binding pattern cannot be regarded as a reliable histochemical marker for the differentiation of MN from MMs, because the pattern reveals no statistically significant correlation with the thickness or the depth of invasion of MM, and seems to lack prognostic significance.
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The data suggest that alteration of complex carbohydrate structures may play a central role in modulating cell-cell and cell-matrix interactions, and that glycosaminoglycans patterns were significantly modified during palatogenesis.
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TLDR
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TLDR
A general reduction of oligosaccharide structures during development of Meckel's cartilage is demonstrated, indicating that intralaminar glucose and/or mannose sequences as well as terminal sialic acid molecules are ubiquitously distributed, while terminal α-fucose was constantly negative.
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TLDR
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The high specificity of the technique is combined with a good sensitivity and resolution as demonstrated by a differential plasma membrane staining in renal epithelial cells.
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