The use of immunoblotting to detect antibodies to nuclear and cytoplasmic antigens. Clinical and serological associations in rheumatic diseases.

@article{Rooij1988TheUO,
  title={The use of immunoblotting to detect antibodies to nuclear and cytoplasmic antigens. Clinical and serological associations in rheumatic diseases.},
  author={Dirk Jan de Rooij and L B van de Putte and W. J. Habets and Andr{\'e} L. M. Verbeek and Walther J. van Venrooij},
  journal={Scandinavian journal of rheumatology},
  year={1988},
  volume={17 5},
  pages={353-64}
}
Using the immunoblotting technique, sera from 433 patients with rheumatic diseases were screened for the presence of antibodies against several nuclear and cytoplasmic antigens, such as RNP, Sm, Ro(SSA), La(SSB), CR-19 (centromeric antigen), Topo-1 (Scl-70), Jo-1, histone and 56 kD. At the same time clinical data from these patients were collected without prior knowledge of the immunoblotting results. Syndrome-specific autoantibodies were found for mixed connective tissue disease (antibodies… CONTINUE READING