The use of hyaluronidase and glucosidase to remove mucus from the rat middle ear cavities for SEM studies

Abstract

To conduct SEM studies on epithelium containing mucus-producing cells it is essential to remove the mucus which normally obscures the epithelial surface. This study presents a method which effectively removes the covering layer of mucus in the rat middle ear. Healthy Sprague-Dawley rats were decapitated and the middle ears dissected free. Incubation and agitation of the middle ear specimens in hyaluronidase (50 IE ml−1) and/or glucosidase (8%) removed the mucus from the middle ear cavity without altering the surface structures. It was also revealed that substances such as polyvinyl-pyrrolidone (PVP) (used to increase the colloid osmotic pressure of, e.g., the fixative solution) must be omitted when preparing ciliated specimens for SEM.

DOI: 10.1007/BF01047466

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Cite this paper

@article{Nilsson1992TheUO, title={The use of hyaluronidase and glucosidase to remove mucus from the rat middle ear cavities for SEM studies}, author={Magnus Nilsson and Sten Olof Hellstr{\"{o}m and Ulla Hedlund}, journal={The Histochemical Journal}, year={1992}, volume={24}, pages={166-169} }