The use of human hepatocyte cultures to study the induction of cytochrome P-450.

@article{Kostrubsky1999TheUO,
  title={The use of human hepatocyte cultures to study the induction of cytochrome P-450.},
  author={Vsevolod E. Kostrubsky and Vinitha Ramachandran and R Venkataramanan and Kenneth Dorko and James E. Esplen and Steven Zhang and Jacqueline F. Sinclair and Steven A. Wrighton and Stephen C. Strom},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={1999},
  volume={27 8},
  pages={887-94}
}
We have previously reported that paclitaxel (Taxol) is a potent inducer of cytochrome P-450 (CYP) 3A protein and CYP3A mRNA in human hepatocyte cultures. Here we report that Taxol increased CYP3A-dependent testosterone 6beta-hydroxylation in intact hepatocytes. This effect was concentration-dependent, with maximal increase in enzyme activity being observed… CONTINUE READING