The use of homocysteine in the estimation of ascorbic acid in urine.

@article{Howard1966TheUO,
  title={The use of homocysteine in the estimation of ascorbic acid in urine.},
  author={Alan Norman Howard and B. J. Constable},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1966},
  volume={13 3},
  pages={
          387-92
        }
}
Abstract The estimation of ascorbic acid in urine is described using a method which has the advantage of being both rapid and specific. Dehydroascorbic acid, formed by shaking urine with charcoal, is re-converted to ascorbic acid by reduction with homocysteine and then titrated against 2,6-dichlorophenolindophenol dye colorimetrically. A measure of interfering substances can be obtained by reduction with homocysteine in the presence of boric acid which inhibits the reduction of dehydroascorbic… CONTINUE READING