The use of heavy nitrogen in quantitative proteomics experiments in plants.

@article{Arsova2012TheUO,
  title={The use of heavy nitrogen in quantitative proteomics experiments in plants.},
  author={Borjana Arsova and Sylwia Kierszniowska and Waltraud X. Schulze},
  journal={Trends in plant science},
  year={2012},
  volume={17 2},
  pages={102-12}
}
In the growing field of plant systems biology, there is an undisputed need for methods allowing accurate quantitation of proteins and metabolites. As autotrophic organisms, plants can easily metabolize different nitrogen isotopes, resulting in proteins and metabolites with distinct molecular mass that can be separated on a mass spectrometer. In comparative quantitative experiments, treated and untreated samples are differentially labeled by nitrogen isotopes and jointly processed, thereby… CONTINUE READING
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