The use of green fluorescent proteins to view association between phospholipase C beta and G protein subunits in cells.

@article{Scarlata2004TheUO,
  title={The use of green fluorescent proteins to view association between phospholipase C beta and G protein subunits in cells.},
  author={Suzanne F. Scarlata and Louisa Dowal},
  journal={Methods in molecular biology},
  year={2004},
  volume={237},
  pages={223-32}
}
A major advance in biology is the ability to attach either green fluorescence protein (GFP) or one of its variants to a target protein and follow its cellular localization and interaction with other partners by fluorescence microscopy. Our laboratory has previously developed fluorescence energy-transfer methods to measure the kinetics and affinities of the lateral association between phospholipase C (PLC) and G protein subunits on membrane surfaces. We are currently developing methods to view… CONTINUE READING

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