The use of fluorogenic substrates to measure fungal presence and activity in soil.

  title={The use of fluorogenic substrates to measure fungal presence and activity in soil.},
  author={Mark Miller and Ansa Paloj{\"a}rvi and A. Rangger and Morten Reeslev and Annelise Helene Kj\oller},
  journal={Applied and environmental microbiology},
  volume={64 2},
Our objective was to determine if 4-methylumbelliferyl-labelled enzyme substrates could be used to detect and quantify specific components of chitinase and cellulase activities as specific indicators of the presence and activity of fungal biomass. The fluorogenic substrates 4-methylumbelliferyl (MUF) N-acetyl-beta-D-glucosaminide and MUF beta-D-lactoside were used for the detection and quantification of beta-N-acetylglucosaminidase (EC (NAGase) and endo 1,4-beta-glucanase (EC… CONTINUE READING
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