The use of fluorescent fragment length analysis (PCR-FFL) in the direct diagnosis and identification of cutaneous Leishmania species.

@article{TomsPrez2013TheUO,
  title={The use of fluorescent fragment length analysis (PCR-FFL) in the direct diagnosis and identification of cutaneous Leishmania species.},
  author={M{\'i}riam Tom{\'a}s-P{\'e}rez and Roser Fisa and Cristina Soler Riera},
  journal={The American journal of tropical medicine and hygiene},
  year={2013},
  volume={88 3},
  pages={586-91}
}
Leishmaniasis is a disease caused by different species belonging to the genus Leishmania. It presents different epidemiological and clinical features and requires the development of rapid, sensitive techniques to improve specific diagnosis. In this study, we compared the traditional technique of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with PCR-fluorescent fragment length analysis (PCR-FFL). Fluorescently tagged primers, designed in the rRNA fragment ITS-1… CONTINUE READING