The use of flow cytometry to discriminate avian lymphocytes from contaminating thrombocytes.

@article{Bohls2006TheUO,
  title={The use of flow cytometry to discriminate avian lymphocytes from contaminating thrombocytes.},
  author={Ryan L Bohls and Roger V. Smith and Pam J Ferro and Nova J. Silvy and Zhongxia Li and Ellen Whited Collisson},
  journal={Developmental and comparative immunology},
  year={2006},
  volume={30 9},
  pages={843-50}
}
Evaluation of peripheral blood mononuclear cells (PBMC) in avian species by flow cytometry is complicated by the presence of large numbers of nucleated thrombocytes. With light scattering properties similar to those of lymphocytes, variations in the proportion of thrombocytes in PBMC can result in apparent shifts in percentages of lymphocyte subpopulations. We have applied a dual-labeling procedure for flow cytometric analyses to exclude thrombocytes from the analyzed population by labeling… CONTINUE READING

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