The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

@article{Niesen2007TheUO,
  title={The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability},
  author={Frank H. Niesen and Helena Berglund and Masoud Vedadi},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={2212-2221}
}
Differential scanning fluorimetry (DSF) is a rapid and inexpensive screening method to identify low-molecular-weight ligands that bind and stabilize purified proteins. The temperature at which a protein unfolds is measured by an increase in the fluorescence of a dye with affinity for hydrophobic parts of the protein, which are exposed as the protein unfolds. A simple fitting procedure allows quick calculation of the transition midpoint; the difference in the temperature of this midpoint in the… Expand
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