The use of antiavidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase technics.

@article{Hsu1981TheUO,
  title={The use of antiavidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase technics.},
  author={Siu Man Hsu and Laurence M. Raine and Herbert Fanger},
  journal={American journal of clinical pathology},
  year={1981},
  volume={75 6},
  pages={
          816-21
        }
}
Avidin has an extraordinary affinity for the small-molecule vitamin biotin. Covalently coupling biotin or avidin to peroxidase molecules does not interfere with their normal biochemical functions. The avidin or biotin molecules, either peroxidase conjugated or unconjugated, can be brought to the antigen sites by means of an antiavidin antibody. Several immunohistochemical staining technics based on this principal have been described. The method utilizing an avidin-biotin-peroxidase complex was… CONTINUE READING
BETA

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 22 CITATIONS

Similar Papers

Loading similar papers…