The use of aluminium lake of nuclear fast red in plant material succesively with alcian blue

@article{Benes2008TheUO,
  title={The use of aluminium lake of nuclear fast red in plant material succesively with alcian blue},
  author={Karel Benes and Miroslav Kam{\'i}nek},
  journal={Biologia Plantarum},
  year={2008},
  volume={15},
  pages={294-297}
}
The successive staining alcian blue/aluminium lake of nuclear fast red was proved a useful tool for studies on plant root tip. A simple and reliable procedure is given resulting in blue cell walls, almost colourless cytoplasm and red nuclei. Attempts were made to apply spectrophotometry and paper chromatography to overcome the confusions in manufacturers’ labelling of the dye and to check the lake formation.