The use of a laser for correlating light and electron microscopic images in thick tissue specimens.


Correlating images between light and electron microscopy is difficult especially in tissue specimens with a substantial z-dimension. To facilitate correlated light and electron microscopy (CLEM) in thick tissue specimens, we describe a basic method of using a femto-pulsed near-infrared laser to burn precise three-dimensional fiducial markers that… (More)
DOI: 10.1016/B978-0-12-801075-4.00015-X