The use of Yarrowia lipolytica for the expression of human cytochrome P450 CYP1A1.

@article{Nthangeni2004TheUO,
  title={The use of Yarrowia lipolytica for the expression of human cytochrome P450 CYP1A1.},
  author={M B Nthangeni and Philippe Urban and Denis Pompon and Martha Sophia Smit and J-M Nicaud},
  journal={Yeast},
  year={2004},
  volume={21 7},
  pages={583-92}
}
Cytochromes P450 constitute a superfamily of haem-thiolate mono-oxygenases that are involved in the oxidative metabolism of lipophilic subtrates. These enzymes require association with cytochrome P450 reductase (CPR) to achieve optimal activities. We have expressed human cytochrome P450 CYP1A1 under the POX2 promoter (pPOX2-CYP1A1) in Y. lipolytica, with or without overproduction of Y. lipolytica CPR expressed under the ICL promoter (pICL-CPR) or the POX2 promoter (pPOX2-CPR). Activity of… CONTINUE READING