The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.

@article{Desai1998TheUO,
  title={The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.},
  author={Amar A. Desai and Annamarie Murray and Timothy J Mitchison and Claire E Walczak},
  journal={Methods in cell biology},
  year={1998},
  volume={61},
  pages={
          385-412
        }
}
Publisher Summary This chapter presents detailed methods for the preparation of cytostatic factor (CSF) extracts and for performing spindle assembly reactions. It also describes methods for depleting specific components from extracts, an approach that has been used successfully to determine the contributions of both motor and nonmotor components to spindle assembly. It also describes methods for analyzing anaphase in vitro. Mature Xenopus eggs are arrested in metaphase of meiosis II by CSF… CONTINUE READING

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