The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitfly.

@article{Takasu2014TheUO,
  title={The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitfly.},
  author={Yoko Takasu and Toshiki Tamura and Suresh Sajwan and Isao Kobayashi and Michal Žurovec},
  journal={Methods},
  year={2014},
  volume={69 1},
  pages={46-57}
}
Transcription activator-like effector nucleases (TALENs) are custom-made enzymes designed to cut double-stranded DNA at desired locations. The DNA breaks are repaired either by error-prone non-homologous end-joining (NHEJ) pathway or via homologous recombination requiring homologous DNA as a template for the repair. TALENs are used for site-specific mutagenesis in an extended range of organisms including insects. We will describe here a simple TALEN-based mutagenesis protocol suitable for the… CONTINUE READING
Related Discussions
This paper has been referenced on Twitter 2 times. VIEW TWEETS

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

Genome engineering: Drosophila melanogaster and beyond.

Wiley interdisciplinary reviews. Developmental biology • 2016

Similar Papers

Loading similar papers…