The use of Renilla luciferase, Oplophorus luciferase, and apoaequorin as bioluminescent reporter protein in the presence of coelenterazine analogues as substrate.

@article{Inouye1997TheUO,
  title={The use of Renilla luciferase, Oplophorus luciferase, and apoaequorin as bioluminescent reporter protein in the presence of coelenterazine analogues as substrate.},
  author={Shigeharu Inouye and Osamu Shimomura},
  journal={Biochemical and biophysical research communications},
  year={1997},
  volume={233 2},
  pages={
          349-53
        }
}
To investigate the use of various luciferases as reporter protein, the substrate specificity of recombinant Renilla luciferase, Oplophorus luciferase and recombinant apoaequorin was examined using 23 kinds of coelenterazine analogues as the substrate. The intensity of luminescence was generally highest with Oplophorus luciferase and lowest with apoaequorin, but varied widely by the substrate used. A very high level of light intensity was obtained when the luminescence reactions of e… CONTINUE READING
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