The use of NASBA for the detection of microbial pathogens in food and environmental samples.

@article{Cook2003TheUO,
  title={The use of NASBA for the detection of microbial pathogens in food and environmental samples.},
  author={Nigel T. Cook},
  journal={Journal of microbiological methods},
  year={2003},
  volume={53 2},
  pages={165-74}
}
  • Nigel T. Cook
  • Published 2003 in Journal of microbiological methods
The isothermal amplification method nucleic acid sequence-based amplification (NASBA), which amplifies RNA, has been reported as useful for the detection of microbial pathogens in food and environmental samples. Methods have been published for Campylobacter spp., Listeria monocytogenes and Salmonella enterica ser. Enteritidis in various foods and for Cryptosporidium parvum in water. Both 16S rRNA and various mRNAs have been used as target molecules for detection; the latter may have advantages… CONTINUE READING

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