The use of HLA-A*0201-transfected K562 as standard antigen-presenting cells for CD8(+) T lymphocytes in IFN-gamma ELISPOT assays.

@article{Britten2002TheUO,
  title={The use of HLA-A*0201-transfected K562 as standard antigen-presenting cells for CD8(+) T lymphocytes in IFN-gamma ELISPOT assays.},
  author={Cedrik M. Britten and Ralf Georg Meyer and Tanja Kreer and Ingo Drexler and Thomas Woelfel and Wolfgang Herr},
  journal={Journal of immunological methods},
  year={2002},
  volume={259 1-2},
  pages={95-110}
}
ELISPOT assays are increasingly used for a direct detection and quantification of single antigen-specific T cells in freshly isolated peripheral blood mononuclear cells (PBMC). They are particularly attractive for the monitoring of specific T lymphocyte responses in clinical trials assessing antigen-specific immunizations in patients with cancer or chronic viral infections. However, one major limitation for the broad clinical implementation of ELISPOT assays is the lack of an inexhaustible… CONTINUE READING
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