The ultrastructure of yeast: cell wall structure and formation.

@article{Osumi1998TheUO,
  title={The ultrastructure of yeast: cell wall structure and formation.},
  author={Masako Osumi},
  journal={Micron},
  year={1998},
  volume={29 2-3},
  pages={
          207-33
        }
}
  • M. Osumi
  • Published 1 April 1998
  • Biology
  • Micron
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doi.org
281 Citations
Highly Influential Citations
15
Background Citations
106
Methods Citations
16
Results Citations
9

281 Citations

On the Ultrastructure of Yeast Cells
TLDR
It is concluded that the actin cytoskeleton governs septum formation during cell division, and the cell wall biogenesis of the fission yeast Schizosaccharomyces pombe was defined using this technique.
Ultrastructural Studies on Cell Wall and Septum Formation in Fission Yeast
TLDR
It was confirmed that the secreted glucans of fission yeast stretch into fibrils and then pack into bundles, which are oriented in parallel and connected in a side-by-side manner to develop wide ribbon-like structures, and it was suggested that the wide glucan fibril function to maintain the cell wall integrity by forming the network structures.
Visualization of yeast cells by electron microscopy.
  • M. Osumi
  • Biology
    Journal of electron microscopy
  • 2012
TLDR
The dynamic process of cell wall formation was clarified through the study of the protoplasts, and it was found that β-1,3-glucan, β,1,6- glucan and α-1+, as well as α-galactomannan, are ingredients of the cell wall.
Ultrastructure and behavior of actin cytoskeleton during cell wall formation in the fission yeast Schizosaccharomyces pombe.
TLDR
A filasome is one of the F-actin patch structures appearing in the cytoplasm at the site of the initial formation of the cell wall and it may play an important role in this action.
[Dynamics of cell wall formation from Trichophyton mentagrophytes protoplast].
  • M. Osumi
  • Biology
    Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology
  • 1998
TLDR
A model of the cell wall composition of Trichophyton is proposed based on the ultrastructure of the mycelial surface of T. rubrum with lanoconazole and the dynamics of the regeneration of this protoplast and the formation of cell wall using ultrahigh resolution low-voltage scanning electron microscopy and transmission electron microscope (TEM).
2015A Frankl Microbial Cell
TLDR
The goal of this review is to guide researchers that want to investigate a particular process at the ultrastructural level in yeast by aiding in the selection of the most appropriate approach to visualize a specific structure or subcellular compartment.
Morphogenesis of the Fission Yeast Cell through Cell Wall Expansion
Fission yeast cell wall biosynthesis and cell integrity signalling
Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens
TLDR
A model for eisosome formation is proposed wherein positively charged recognition patches first establish contact with target membrane regions and a (partial) unwinding of the coiled-coil conformation of the BAR domains then allows interactions between the hydrophobic faces of their amphipathic helices and the lipid phase of the inner membrane leaflet, generating the striated patterns.
The Cell Wall of Filamentous Fungi
TLDR
The filamentous fungal cell wall is adapted to extremely rapid deposition and growth at the hyphal tip and an ability to penetrate hard surfaces, and the significance of the cell wall as a target for antifungals has long been appreciated.
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References

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Cell wall formation in regenerating protoplasts of Schizosaccharomyces pombe: study by high resolution, low voltage scanning electron microscopy.
TLDR
It is suggested that beta-glucan is the main component of the microfibrils and that it plays an important role in the formation of the cell wall in S. pombe.
Morphological aspects of cell wall formation during protoplast regeneration in Candida albicans.
TLDR
The dynamics of cell wall regeneration in Candida albicans protoplasts was investigated using a new fluorescent dye, Fungiflora Y, which specifically binds to beta-linked polysaccharides, as well as by electron microscopy.
Transmission and scanning electron microscopic examination of intracellular organelles in freeze-substituted Kloeckera and Saccharomyces cerevisiae Yeast Cells
TLDR
In the cytological analysis of the yeasts, intracellular structures were well preserved by using the freeze-substitution fixation method, and the previous model of a yeast cell was modified.
STUDIES ON THE CYTOLOGICAL STRUCTURE OF YEAST: ELECTRON MICROSCOPY OF THIN SECTIONS
TLDR
The cytoplasmic structures observed in sections of cells grown on Lindegren's presporulation medium are the subject of this paper.
Three-dimensional analysis of morphogenesis induced by mating pheromone alpha factor in Saccharomyces cerevisiae.
TLDR
Ultrastructural analyses of cytoplasmic changes in Saccharomyces cerevisiae X2180-1A (MATa) that had been treated with alpha factor were performed by using the freeze-substitution fixation method, showing a polar organization of the cy toplasm during projection formation.
Ultrastructural analysis of the autophagic process in yeast: detection of autophagosomes and their characterization
TLDR
Results indicated that spherical structures completely enclosed in a double membrane were found near the vacuoles of protease-deficient mutant cells when the cells were shifted to nutrient-starvation media, suggesting that these structures are precursors of autophagic bodies, "autophagosomes" in yeast.
Ultrahigh-resolution low-voltage SEM reveals ultrastructure of the glucan network formation from fission yeast protoplast.
TLDR
Improved ultrahigh-resolution low-voltage SEM (UHR-LVSEM) images of reverting protoplasts treated with glucanase confirmed that the particles scattered on the protoplast surface in the initial stage of regeneration were glucan in nature.
Ultracytochemical evidence of Golgi functions in microvesicles at all phases of cell cycle in Saccharomyces cerevisiae.
Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces
TLDR
The distribution of actin and tubulin during the cell cycle of the budding yeast Saccharomyces was mapped by immunofluorescence using fixed cells from which the walls had been removed by digestion to suggest that it may have a role in the localized deposition of new cell wall material.
Actin is associated with the formation of the cell wall in reverting protoplasts of the fission yeast Schizosaccharomyces pombe.
TLDR
It is demonstrated that actin is associated with initiation of cell wall formation, the proper deposition ofcell wall materials, and maintaining the normal morphology of reverting protoplasts.
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