281 Citations
On the Ultrastructure of Yeast Cells
- Biology
- 2002
It is concluded that the actin cytoskeleton governs septum formation during cell division, and the cell wall biogenesis of the fission yeast Schizosaccharomyces pombe was defined using this technique.
Ultrastructural Studies on Cell Wall and Septum Formation in Fission Yeast
- Biology
- 2005
It was confirmed that the secreted glucans of fission yeast stretch into fibrils and then pack into bundles, which are oriented in parallel and connected in a side-by-side manner to develop wide ribbon-like structures, and it was suggested that the wide glucan fibril function to maintain the cell wall integrity by forming the network structures.
Visualization of yeast cells by electron microscopy.
- BiologyJournal of electron microscopy
- 2012
The dynamic process of cell wall formation was clarified through the study of the protoplasts, and it was found that β-1,3-glucan, β,1,6- glucan and α-1+, as well as α-galactomannan, are ingredients of the cell wall.
Ultrastructure and behavior of actin cytoskeleton during cell wall formation in the fission yeast Schizosaccharomyces pombe.
- BiologyJournal of electron microscopy
- 2003
A filasome is one of the F-actin patch structures appearing in the cytoplasm at the site of the initial formation of the cell wall and it may play an important role in this action.
[Dynamics of cell wall formation from Trichophyton mentagrophytes protoplast].
- BiologyNihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology
- 1998
A model of the cell wall composition of Trichophyton is proposed based on the ultrastructure of the mycelial surface of T. rubrum with lanoconazole and the dynamics of the regeneration of this protoplast and the formation of cell wall using ultrahigh resolution low-voltage scanning electron microscopy and transmission electron microscope (TEM).
2015A Frankl Microbial Cell
- Biology
- 2015
The goal of this review is to guide researchers that want to investigate a particular process at the ultrastructural level in yeast by aiding in the selection of the most appropriate approach to visualize a specific structure or subcellular compartment.
Morphogenesis of the Fission Yeast Cell through Cell Wall Expansion
- Biology, EngineeringCurrent Biology
- 2015
Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens
- BiologyEukaryotic Cell
- 2015
A model for eisosome formation is proposed wherein positively charged recognition patches first establish contact with target membrane regions and a (partial) unwinding of the coiled-coil conformation of the BAR domains then allows interactions between the hydrophobic faces of their amphipathic helices and the lipid phase of the inner membrane leaflet, generating the striated patterns.
The Cell Wall of Filamentous Fungi
- Biology
- 2010
The filamentous fungal cell wall is adapted to extremely rapid deposition and growth at the hyphal tip and an ability to penetrate hard surfaces, and the significance of the cell wall as a target for antifungals has long been appreciated.
References
SHOWING 1-10 OF 60 REFERENCES
Cell wall formation in regenerating protoplasts of Schizosaccharomyces pombe: study by high resolution, low voltage scanning electron microscopy.
- BiologyJournal of electron microscopy
- 1989
It is suggested that beta-glucan is the main component of the microfibrils and that it plays an important role in the formation of the cell wall in S. pombe.
Morphological aspects of cell wall formation during protoplast regeneration in Candida albicans.
- BiologyJournal of electron microscopy
- 1995
The dynamics of cell wall regeneration in Candida albicans protoplasts was investigated using a new fluorescent dye, Fungiflora Y, which specifically binds to beta-linked polysaccharides, as well as by electron microscopy.
Transmission and scanning electron microscopic examination of intracellular organelles in freeze-substituted Kloeckera and Saccharomyces cerevisiae Yeast Cells
- Biology
- 1987
In the cytological analysis of the yeasts, intracellular structures were well preserved by using the freeze-substitution fixation method, and the previous model of a yeast cell was modified.
STUDIES ON THE CYTOLOGICAL STRUCTURE OF YEAST: ELECTRON MICROSCOPY OF THIN SECTIONS
- BiologyJournal of bacteriology
- 1957
The cytoplasmic structures observed in sections of cells grown on Lindegren's presporulation medium are the subject of this paper.
Three-dimensional analysis of morphogenesis induced by mating pheromone alpha factor in Saccharomyces cerevisiae.
- BiologyJournal of cell science
- 1989
Ultrastructural analyses of cytoplasmic changes in Saccharomyces cerevisiae X2180-1A (MATa) that had been treated with alpha factor were performed by using the freeze-substitution fixation method, showing a polar organization of the cy toplasm during projection formation.
Ultrastructural analysis of the autophagic process in yeast: detection of autophagosomes and their characterization
- BiologyThe Journal of cell biology
- 1994
Results indicated that spherical structures completely enclosed in a double membrane were found near the vacuoles of protease-deficient mutant cells when the cells were shifted to nutrient-starvation media, suggesting that these structures are precursors of autophagic bodies, "autophagosomes" in yeast.
Ultrahigh-resolution low-voltage SEM reveals ultrastructure of the glucan network formation from fission yeast protoplast.
- EngineeringJournal of electron microscopy
- 1995
Improved ultrahigh-resolution low-voltage SEM (UHR-LVSEM) images of reverting protoplasts treated with glucanase confirmed that the particles scattered on the protoplast surface in the initial stage of regeneration were glucan in nature.
Ultracytochemical evidence of Golgi functions in microvesicles at all phases of cell cycle in Saccharomyces cerevisiae.
- BiologyMicron
- 1995
Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces
- BiologyThe Journal of cell biology
- 1984
The distribution of actin and tubulin during the cell cycle of the budding yeast Saccharomyces was mapped by immunofluorescence using fixed cells from which the walls had been removed by digestion to suggest that it may have a role in the localized deposition of new cell wall material.
Actin is associated with the formation of the cell wall in reverting protoplasts of the fission yeast Schizosaccharomyces pombe.
- BiologyJournal of cell science
- 1989
It is demonstrated that actin is associated with initiation of cell wall formation, the proper deposition ofcell wall materials, and maintaining the normal morphology of reverting protoplasts.