The ultrastructure of the chromosome periphery in human cell lines

@article{Gautier1992TheUO,
  title={The ultrastructure of the chromosome periphery in human cell lines},
  author={Thierry Gautier and Claude Masson and C Quintana and Jacqueline Arnoult and Dani{\'e}le Hernandez-Verdun},
  journal={Chromosoma},
  year={1992},
  volume={101},
  pages={502-510}
}
We studied the chromosome periphery in human HeLa and TG cells using cryomethods in electron microscopy. A contrasted layer of peripheral chromosomal material (PCM) was visible in cryo-ultrathin sections of mitotic cells. This PCM was composed of closely packed fibrils associated with granules. The PCM did not cover the entire chromosome surface but was found around most of the chromosomes and even between two chromatids. The organization of the PCM was not affected by colchicine treatment of… CONTINUE READING